Drugs online research references
Res Commun Chem Pathol Pharmacol. 1993 Jan;79(1):117-20.
Effects of 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid, an inhibitor of Cl(-)-HCO3- exchanger, on stress-induced gastric lesions in rats.
Horie S, Yano S, Watanabe K.
Department of Drug Evaluation and Toxicological Sciences, Faculty of Pharmaceutical Sciences, Chiba University, Japan.
The effect of 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS), an inhibitor of Cl(-)-HCO3- exchanger, on stress-induced gastric lesions was studied in rats. The stress exposure to rats was performed by restraint plus water-immersion (22 degrees C) for 6 h. Oral administration of DIDS produced a dose-dependent reduction in the gastric lesion formation. The ED50 value for DIDS was 94.4 mg/kg (64.2 - 138.8 mg/kg; 95% confidence limits) and the maximum effect was observed at the dose of 300 mg/kg with an inhibition of 95%. A dose-dependent reduction in lesions was also observed after administration of omeprazole, an inhibitor of proton pump. The ED50 value for omeprazole was 3.1 mg/kg (1.8 - 5.4 mg/kg; 95% confidence limits) and the effect was maximum at 16 mg/kg with an inhibition of 92%. These results indicated that DIDS protected the gastric mucosa against stress-induced lesions. It is speculated that the antiulcerous activity of DIDS may give a clue for the development of a new class of therapeutic drugs for the prevention and healing of gastric ulcers.
online pharmacy ref source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=8434127&dopt=Abstract
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Eur J Gastroenterol Hepatol. 1997 Jan;9(1):49-53.
Ongoing gastric acid inhibition is a confounding factor in Helicobacter pylori diagnosis.
Jonkers D, Houben P, Stobberingh E, Stockbrugger R.
Department of Gastroenterology, University Hospital Maastricht, University of Limburg, The Netherlands.
BACKGROUND: Eradication of Helicobacter pylori by antibiotics in combination with gastric acid inhibition can result in overgrowth of non-H. pylori bacterial flora. This may confound the histological detection of H. pylori at eradication control if non-specific staining methods are used. OBJECTIVE AND METHODS: In 18 patients treated with amoxycillin (2 weeks) and omeprazole (6 weeks), endoscopically obtained gastric juice was cultured and two biopsies of corpus, antrum and duodenum were taken before and after eradication therapy (with gastric acid inhibition still going on) for culture and for histology to assess the intragastric bacterial flora. By histology, modified Giemsa (MG) and an H. pylori-specific immunohistochemical stain (IMM) were evaluated. RESULTS: Median pH of gastric juice was 1.5 (n = 18) before and 7 (n = 17) after eradication therapy, when patients were still on omeprazole. After therapy, culture showed a significant decrease (P < 0.05) in mean amount of H. pylori in corpus, antral and duodenal biopsies and a significant increase of non-H. pylori flora (P < 0.05) in gastric juice, corpus, antral and duodenal mucosa. With culture as a standard, 16 and 4 biopsy specimens were scored falsely positive for H. pylori by MG and IMM, respectively, and H. pylori was not detected in 23 and 13 biopsy specimens when culture was H. pylori-positive. CONCLUSION: Because of the possible presence of non-H. pylori flora after eradication therapy, the use of IMM is recommended in this situation for the histological detection of H. pylori, especially in those patients with ongoing gastric acid inhibitory therapy.
online pharmacy ref source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=9031899&dopt=Abstract
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Biochem Pharmacol. 1994 Nov 29;48(11):2049-55.
Specific proton pump inhibitors E3810 and lansoprazole affect the recovery process of gastric secretion in rats differently.
Tomiyama Y, Morii M, Takeguchi N.
Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Japan.
After a single subcutaneous administration (30 mg/kg) of proton pump inhibitor 2-[(4-(3-methoxypropoxy)-3-methylpyridin-2-yl)-methylsulfiny l]- 1H-benzimidazole sodium salt (E3810), or lansoprazole in rats, time courses of inhibitory and recovery processes of acid secretion in vivo and pump enzyme activity in isolated microsomes were measured. The acid secretion rate which reflects H+,K(+)-ATPase activity in the secretory canalicular (apical) membrane was compared with that in the microsomal fraction which consists mostly of resting, intracellularly-pooled tubulovesicles. We found that the canalicular pump was first inhibited, followed by slow inhibition of the microsomal pump enzyme activity, with the rate of the latter process depending on the inhibitors. It took 2.5 hr for the half-maximal inhibition of the microsomal pump in E3810-treated rats, and 6 hr in lansoprazole-treated rats. The acid secretion and the microsomal enzyme activity completely recovered within 48 hr after the administration of E3810, but recovered by only 20% even 96 hr after the administration of lansoprazole. Incubation with dithiothreitol of isolated microsomes obtained from E3810-treated rats reactivated the enzyme activity, but not from rats treated with lansoprazole. These results suggest that dissociation of inhibitor from the pump and/or intracellular transport of the pump is affected differently by these inhibitors. Furthermore, it is possible that the half life of the proton pump protein is much longer (greater than 96 hr) than the previously proposed value of 30-48 hr.
online pharmacy ref source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=7802694&dopt=Abstract
note: kwd match prilosec online literature
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