Drugs online research references
Clin Chem. 1988 Nov;34(11):2348-51.
Results compared for tricyclic antidepressants as assayed by liquid chromatography and enzyme immunoassay.
Dorey RC, Preskorn SH, Widener PK.
Department of Chemistry, Wichita State University, KS 67208.
The tricyclic antidepressants amitriptyline, nortriptyline, imipramine, and desipramine in serum of patients taking one of the drugs were quantified in two laboratories by high-performance liquid chromatography (HPLC) and enzyme-multiplied immunoassay (EMIT; Syva). Results for split samples were highly correlated, but EMIT gave higher results in most cases, and the slopes of the correlation lines for each analyte were greater than 1. Detection limits for the two procedures were such that 18% of the EMIT results for the drug(s) were considered negative, as compared with 4% of the HPLC results. Additional assay of desmethyl or hydroxy antidepressant metabolites by HPLC did not explain the higher EMIT results. The relatively high detection limit for EMIT greatly limits its use in therapeutic drug monitoring, where low concentrations of tricyclic antidepressants are as important as high ones for dose adjustment or determination of compliance. Other problems with EMIT measurement of tricyclic antidepressants are discussed.
online pharmacy ref source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=3052930&dopt=Abstract
Scand J Clin Lab Invest. 1980 Apr;40(2):191-7.
Radioimmunoassay for tricyclic antidepressants.
Virtanen R.
A sensitive radioimmunoassay for the determination of tricyclic antidepressants is described. The antibodies were produced in rabbits immunized with a conjugate of N-succinyl-nortriptyline and human serum albumin and they were shown to be specific for the tricyclic antidepressant group of drugs. The assay was tested for the determination of amitriptyline and nortriptyline concentrations in plasma or serum. Specificity was achieved by coupling a simple extraction procedure (based on selective extraction of amitriptyline and nortriptyline at different pH-values) to the assay. Using [3H]imipramine as the tracer the sensitivity of the assay is 3.8-18.0 nmol/l depending on whether the extraction procedure is used or not and it can be carried out with 50-200 microliter sample volumes. Because of its advantages (sensitivity, specificity, simplicity, small sample volumes, low cost) this procedure is suitable for drug monitoring both during controlled tricyclic therapy and pharmacokinetic studies.
online pharmacy ref source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=7256188&dopt=Abstract
Res Commun Chem Pathol Pharmacol. 1986 Nov;54(2):237-53.
Metalloimmunoassay of tricyclic antidepressant drugs: production and characterization of antiserum.
Cheret P, Brossier P.
Metalloimmunoassay is a new immunoassay using an organometallic complex as the label. For the development of this assay, antibodies were produced in rabbits immunized with N-succinyldesipramine-bovine serum albumin conjugate. Antisera thus obtained were shown to be specific for the tricyclic antidepressant group of drugs and are tested as regard as desipramine or nortriptyline labelled by an organometallic moiety (designated metallohaptens). The evaluation of the affinity constants shows that antisera cross-react almost equally well with both imipramine labelled with tritium (radiotracer) and metallohaptens using either ferrocene or cymantrene as labels (metallotracers). Detection of metallohaptens is performed by a ZEEMAN atomic absorption spectrophotometer (ZAAS).
online pharmacy ref source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=3786947&dopt=Abstract
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