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hair related research references
Anal Bioanal Chem. 2002 Sep;374(1):126-30. Epub 2002 Aug 10.
Determination of trace bismuth by flow injection-hydride generation collection-atomic absorption spectrometry.
Chen SY, Zhang ZF, Yu HM.
Center for Physical Sciences, University of Science & Technology of China, Hefei, Anhui, China. chensstc.edu.cn
Bismuth hydride gas was collected on-line and determined via a new flow injection-hydride generation collection-flame atomic absorption spectrometry system. The performance of the gas-liquid separator, hydride gas collection time, acidity of the sample solution, NaBH(4) concentration, and the effects of concomitant interferents were investigated to optimize the conditions of this new method. Interferences from concomitant elements were investigated, and recoveries of 94.7-105.3% for 10 ng mL(-1) Bi were obtained after the addition of 0.2% ascorbic acid-thiourea masking reagents. The sensitivity of this new method was one order of magnitude higher than the continuous flow-hydride generation-flame atomic absorption method with a detection limit of 0.25 ng mL(-1) and a precision of 2.3%. The method was evaluated by determining trace bismuth in standard biological reference material human hair GBW07601, and the results were consistent with the certified value. The proposed method was then employed to determine trace bismuth in ten colored gelatin samples; recoveries of 94.2-105.8% were obtained.
online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12207253&dopt=Abstract
J Chromatogr B Analyt Technol Biomed Life Sci. 2003 Jun 5;789(1):151-67.
Evaluation of electrospray ionisation liquid chromatography-tandem mass spectrometry for rational determination of a number of neuroleptics and their major metabolites in human body fluids and tissues.
Josefsson M, Kronstrand R, Andersson J, Roman M.
National Board of Forensic Medicine, Department of Forensic Chemistry, University Hospital, SE-581 85, Linkoping, Sweden
A study of liquid chromatography-triple quadrupole mass spectrometry (LC-MS-MS) with positive electrospray ionisation (ESI) for the determination of selected drugs in human tissues and body fluids such as blood, urine and hair is described. The possibility to screen for and quantify the 19 most commonly prescribed neuroleptics on the Swedish market and determine the presence of their major metabolites within a single LC-MS-MS analysis was evaluated on a PE Sciex API2000 instrument. Chromatographic conditions were optimised and the best separation, with individual retention times for most of the analytes, was obtained on a Zorbax SB-CN column within a 9-min gradient run. The MS-MS fragmentation conditions were optimised for each compound in order to obtain both specific fragments and high signal intensity. Since neuroleptics are a heterogeneous group of compounds, a markedly difference in collision energy needed to achieve fragments of the selected parent ions was seen and the number of fragments achieved varied as well. For sensitive quantification the transition of the most intense fragment of the protonated molecular ion (M+1)(+) was selected for multiple reaction monitoring analysis. More than 70 transitions were finally included in the assay. Detection levels down to the lower ng/ml level were achieved for all analytes, but between analytes more than a 10-fold difference in signal response was seen. By evaluation of extracted ion chromatograms from the analysis of authentic human blood, urine and hair sample the proposed concept for rational drug analysis was found to be both selective and sensitive for the neuroleptics included. A great number of metabolites could be determined in blood, urine and hair as well. A full method validation was not performed since the objective was to evaluate the method design rather than to validate a final method set-up.
online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12726853&dopt=Abstract [PubMed - in process]
Acta Anat (Basel). 1979;103(4):434-44.
Melanin and dopa-positive cells in the skin of tropical cattle.
Amakiri SF.
Various histochemical and histological techniques were used to study the melanin and dopa-positive cell distribution in the skin of some tropical and temperate breeds of cattle in Nigeria. Melanin pigments were concentrated in the basal and lower spinous layers of the epidermis and in the hair cortex, follicle sheaths and papillae of the various breeds. In the White Fulani and N'Dama breeds, melanin pigments were however found in all layers of the epidermis. Dopa-positive cells (melanocytes) were observed in the epidermis, dermis and hair follicles; the distribution pattern varied among breeds, being copiously disposed in the basal epidermis and papillary dermis in the White Fulani and Muturu and, except in areas of thick epidermal ridges, scanty in the epidermis and dermis of the Friesian and N'Dama. Mast cell distribution pattern in the various breeds was similar to that of the dopa-positive cells. Peroxidase-positive cells were present in the basal epidermis and upper dermis of the Muturu, widespread in the subepidermal layer of the N'Dama and very scanty in the dermis of the White Fulani and Friesian. Acid phosphatase activity was intense in the granular layer of the Muturu and N'Dama breeds and also in the papillary dermis and hair follicles, whereas alkaline phosphatase-positive dendritic cells, and 'clear' cells were also observed in the basal and upper epidermis.
online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=87102&dopt=Abstract
J Cosmet Sci. 2003 May-Jun;54(3):271-81.
UV radiation: aggressive agent to the hair--AFM, a new methodology of evaluation.
Monteiro VF, Pinheiro AS, Leite ER, Agnelli JA, Pereira-Da-Silva MA, Longo E.
CMDMC/LIEC/DQ/UFSCar, Universidade Federal de Sao Carlos, Rod Washington Luis Km 235, Caixa Postal, 676, Sao Carlos, SP, Brazil 13565-905.
A new method for morphological hair analysis at high resolution and under ambient conditions is presented in this paper. The AFM has been used in these experiments to analyze morphological changes in hair roughness and thickness after UV radiation. Through the powerful analytical AFM tools, changes in hair morphology can be proven. A new quantitative methodology to evaluate hair structure is presented in this paper.
online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12858226&dopt=Abstract [PubMed - in process]
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