DreamPharm Products:
Anticancer Res. 2000 Sep-Oct;20(5A):3265-71.
Analysis of the in vitro inhibition of mammary adenocarcinoma cell adhesion by sulphated polysaccharides.
Liu JM, Haroun-Bouhedja F, Boisson-Vidal C.
Laboratoire de Recherche sur les Macromolecules (URM 7540, URM2, CNRS/IFREMER) Institut Galilee, Universite Paris 13, Villetaneuse, France.
Evidence is mounting that changes in the ability of cancer cells to adhere to extracellular matrices (ECM) play a decisive role in metastasis spread. We have investigated the effect of different sulphated polysaccharides on the adhesion of MCF7 and MDA-MB231 adenocarcinoma breast cells to different substrata: a reconstituted basement membrane (Matrigel) and various adhesion-mediating proteins (fibronectin, laminin, type IV collagen). Most of them inhibited cell adhesion and the most active component is a galactose rich units polysaccharide, carrageenan iota. Taken together, the results suggest that this inhibitory activity depends on the charge density related to sulphate groups, the molecular weight and also the carbohydrate structure. These products very likely unstabilize the interaction between the glucosaminoglycan portion of proteoglycans and the ECM proteins and then block the ability of these adhesive proteins to bind to cells.
online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11062752&dopt=Abstract
FEBS Lett. 2002 Nov 20;531(3):505-8.
An enhancer complex confers both high-level and cell-specific expression of the human type X collagen gene.
Chambers D, Young DA, Howard C, Thomas JT, Boam DS, Grant ME, Wallis GA, Boot-Handford RP.
Wellcome Trust Centre for Cell-Matrix Research, School of Biological Sciences, 2.205 Stopford Building, Oxford Road, University of Manchester, M13 9PT, Manchester, UK.
Type X collagen expression is restricted to hypertrophic chondrocytes in the endochondral growth plate. Transient transfection of reporter constructs containing the human collagen X promoter into primary growth plate chondrocytes identified a cis-acting positive regulatory DNA element(s) that has cell-specific enhancer properties and binds a nuclear protein expressed specifically in growth plate chondrocytes. Functional disruption of this region results in a significant reduction in the activation of reporter gene transcription. The identified enhancer is a major element controlling both high-level and cell-specific expression of type X collagen gene.
online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12435601&dopt=Abstract
J Antimicrob Chemother. 2000 Nov;46(5):751-6.
Efficacy of polycationic peptides in preventing vascular graft infection due to Staphylococcus epidermidis.
Giacometti A, Cirioni O, Ghiselli R, Goffi L, Mocchegiani F, Riva A, Scalise G, Saba V.
Institute of Infectious Diseases and Public Health and Department of General Surgery, INRCA IRCCS, University of Ancona, Ancona, Italy. cmalinopcsi.unian.it
A rat model was used to investigate the efficacy of two polycationic peptides, ranalexin and buforin II, in the prevention of vascular prosthetic graft infection due to methicillin-resistant Staphylococcus epidermidis with intermediate resistance to glycopeptides. The in vitro activity of the peptides was compared with those of vancomycin and teicoplanin by MIC determination and time-kill study. Moreover, the efficacy of collagen-sealed peptide-soaked Dacron was evaluated in a rat model of graft infection. Graft infections were established in the dorsal subcutaneous tissue of 120 adult male Wistar rats. The in vivo study included a control group, one contaminated group that did not receive any antibiotic prophylaxis and four contaminated groups that received an antibiotic-soaked graft. Experiments demonstrated that the activities of buforin II and ranalexin were greater than those of vancomycin and teicoplanin. Particularly, rats with buforin II-coated Dacron grafts showed no evidence of staphylococcal infection while, for the rats with ranalexin-, vancomycin- and teicoplanin-coated Dacron grafts, the quantitative graft cultures demonstrated bacterial growth (1.9 x 10(2) +/- 0.6 x 10(2) cfu/mL, 6. 2 x 103 +/- 1.9 x 10(3) cfu/mL and 5.1 x 10(4) +/- 4.8 x 10(3) cfu/mL, respectively). The study demonstrated that the use of peptide-soaked Dacron graft can result in significant bacterial growth inhibition and indicates that these compounds may be potentially useful in prosthetic surgery.
online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11062194&dopt=Abstract
Knee Surg Sports Traumatol Arthrosc. 2000;8(5):281-5.
Type V collagen is increased during rabbit medial collateral ligament healing.
Niyibizi C, Kavalkovich K, Yamaji T, Woo SL.
Musculoskeletal Research Center, Department of Orthopaedic Surgery, Presbyterian-University Hospital, University of Pittsburgh School of Medicine, PA 15213, USA. niyitt.edu
To understand the reparative process of medial collateral ligament (MCL), fibrillar collagen and their relative ratios in healing MCL with anterior cruciate ligament (ACL) reconstruction were analyzed. Skeletally mature New Zealand white rabbits were subjected to a mop-end tear of MCL without repair with ACL reconstruction. Rabbits were killed 6 and 52 weeks after injury. Ligamentous tissues from the injury site and sham controls were soaked in 0.5 M acetic acid for 24 h, minced, and treated with pepsin to solubilize collagen. Pepsin solubilized about 80% of the total collagen as determined by hydroxyproline analysis of the pepsin residues. Sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis of the solubilized collagen revealed presence of fibrillar collagen types I, III, and V. Densitometric scanning of the protein bands corresponding to types I, III, and V collagen indicated that in sham controls types III and V collagen represented about 8% and 12%, respectively, of the type I collagen whereas the healed MCL ligaments at 6 weeks showed significant increase in type III and V collagen to about 19% and 24%, respectively. By 52 weeks type III collagen in the healed MCL had returned to that of sham controls while type V collagen remained elevated at approximately 18%. These data suggest that presence of type V collagen in high concentration in healing ligaments may have an influence on collagen fibril diameters seen in healed ligament and should be included in the analysis when evaluating ligament healing.
online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11061296&dopt=Abstract
Spine. 2000 Nov 1;25(21):2736-41.
Observations on fiber-forming collagens in the anulus fibrosus.
Schollmeier G, Lahr-Eigen R, Lewandrowski KU.
Department of Surgery, Faculty of Medicine, University of Ottawa, Ontario, Canada.
STUDY DESIGN: The spatial distribution of fiber-forming collagens in the anulus fibrosus was investigated in the complete longitudinal and horizontal sections of human lumbar intervertebral discs of seven individuals. OBJECTIVES: To obtain a more detailed structural definition of the anulus fibrosus because structural alterations of its collagen fiber network have been implicated in discal degeneration and other spinal pathologies. SUMMARY OF BACKGROUND DATA: Prior biochemical or immunofluorescence studies permitted only limited conclusions concerning the spatial distribution of the fiber-forming collagens in relation to anatomic structures because they were based on intraoperative tissue specimens or performed on incomplete sections of human intervertebral discs. METHODS: Complete human intervertebral discs with their adjacent vertebral bodies were fixed, decalcified, and embedded in paraffin. The intervertebral disc and its adjacent structures were reviewed in their entirety on one histologic slide. Monoclonal antibodies against human Types I, II, and III collagen were used for immunohistochemistry. A comparative analysis based on both immunohistochemical and histologic evaluation was performed. RESULTS: Type I collagen was seen abundantly in the outer zone and outer lamellas of the inner zone of the anulus fibrosus. On longitudinal sections, the Type I collagen distribution took the shape of a wedge. On horizontal sections, the Type I collagen positive area took the shape of a ring that was wider anteriorly than posteriorly. This suggests that the three-dimensional shape of the Type I collagen-positive tissue in the anulus fibrosus can be described by a donut that is wider anteriorly than posteriorly. Type II collagen was present in the entire inner of the anulus fibrosus, but not in the outer zone. In addition, it was found in the cartilaginous endplates. Type III collagen showed some codistribution with Type II collagen, particularly in pericellular locations in areas of spondylosis, which was noted at the endplates, vertebral rim, and insertion sites of the anulus fibrosus. CONCLUSIONS: These observations on the location of Types I and II collagen provide a more detailed structural definition of the anulus fibrosus, which may assist in further investigation of discal herniation.
online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11064517&dopt=Abstract
Natural Herbal Supplement: Hair Million
Hair loss alone does not pose significant health problems. In fact, there are people who opt for baldness as an alternative hair style. However, in general, however, hair loss is not considered desirable.
The most ostensive feature that distinguishes us human from chimps and other primates is the lack of bodily hair. During evolutionary process, we have lost the majority of hair. Hair is no longer a biologically essential part of our body, just
like appendix. The hair we still have on our scalp and a few other bodily parts is still regarded as significant for reasons other than biological necessity. Hair loss is naturally accompanied by aging process, although the extent of hair loss and the timing of onset vary widely among individuals. Thus, loss of hair and baldness is considered as a symbol of maturity or old age. Like winkles and other signs of aging, hair loss is not welcome by most people, because we don't welcome aging, and being perceived as an aging person. However, it is alopecia, or premature hair loss that especially concerns certain people.
While the hair loss and resulting baldness in general have not been proven to be related to underlying health problems, there are certain correlations between hair loss and health problems. For instance, premature hair loss could suggest premature aging or nutritional and hormonal imbalance, stressful life, use of drugs that cause hair loss as a side effect, skin disease, or heart disease. The balding appearance could also impart a subdued impression of integrity in bodily health and youthfulness.
Fortunately, in many cases, hair loss is reversible by change in lifestyle and/or nutritional supplementation. Herbal hair growth formula and other nutritional supplements have been shown to be effective in warding off hair loss and resuming hair growth. Certain prescription drugs such as Propecia may also reverse hair loss by blocking the formation of DHT, a hormonal byproduct produced inceasingly as a person age.
LipitorTramadol
DHEA has been suggested to provide numerous potential benefits. DHEA (or dehydroepiandrosterone) is converted into androgens (male hormones)
or estrogens (female hormones) in the cells.
Our bodies produce decreasing amount of DHEA as we get older.
various health benefits: To deter aging,
improve sexual function/erectile dysfunction, treat cognitive decline, enhance athletic performance,
facilitate weight loss, improve strength, prevent osteoporosis, enhance immunomodulation for rheumatic conditions,
and treat depression.
Natural Herbal Supplements||
Constipation relief, laxative, colon cleansing ||
Best Realtor in Glendale, California: Residential Home and Commercial Property ||
Related Web pages ||
Viagra ||
Herbs and Pharmaceuticals Online ||
DreamPharm Rx: Buy Xenical Online ||